| Artist | Album | Song Title | Start/Stop | Scene |
| 2x01 - Raindrops Keep Falling On My Head: |
| Bow Wow Wow | I Want Candy | | 00:00:00 - 00:01:50 (DVD) | At the beginning of the episode. |
| Blue-Eyed Son | West of Lincoln | | 00:01:50 - 00:03:47 (DVD) | When Derek and Addison talk at the beginning of the episode. |
| Blue-Eyed Son | West of Lincoln | | 00:04:44 - 00:05:21 (DVD) | When Joe the bartender collapses. |
| FirstCom Music | | | 00:05:26 - 00:06:02 (DVD) | When Meredith and Cristina examine Joe in the bar. |
| Mike Doughty | Haughty Melodic | Looking at the World from the Bottom of a Well | 00:07:37 - 00:08:15 (DVD) | When Meredith is leaving the hospital and Derek tries to explain. |
| Mike Doughty | Haughty Melodic | Looking at the World from the Bottom of a Well | 00:09:18 - 00:09:43 (DVD) | After Derek talked to Richard. |
| Esthero | Wikked Lil' Grrrls | | 00:21:05 - 00:22:37 (DVD) | When George tells Meredith about Burke and Cristina kissing. |
| Ivy | In the Clear | | 00:28:35 - 00:30:26 (DVD) | When Burke and Derek are performing the stand still surgery (on Joe). |
| Joe Purdy | Only Four Seasons | | 00:39:09 - 00:42:24 (DVD) | At the end of the episode. |
| 2x02 - Enough Is Enough (No More Tears): |
| KT Tunstall | Eye To The Telescope | | 00:00:40 - 00:02:59 (DVD) | At the beginning of the episode. |
| Jem | Finally Woken | | 00:13:12 - 00:14:25 (DVD) | When Meredith, Cristina, and Alex are talking about Judy Dolls. |
| Joy Zipper | Heartlight Set | | 00:23:20 - 00:25:12 (DVD) | When the interns are eating lunch. |
| Goldfrapp | Supernature | | 00:30:19 - 00:32:29 (DVD) | When George tells Derek the donor is decerebrate. |
| Brandi Carlile | Brandi Carlile | | 00:39:34 - 00:42:19 (DVD) | At the end of the episode. |
| 2x03 - Make Me Lose Control: |
| Get Set Go | Ordinary World | | 00:00:45 - 00:03:20 (DVD) | At the beginning of the episode. |
| Sylvie Lewis | Tangos and Trantrums | | 00:16:18 - 00:17:47 (DVD) | When Meredith and Alex are talking to Kelly. |
| Roisin Murphy | Ruby Blue | | 00:20:53 - 00:23:54 (DVD) | When Cristina collapses in the operating room. |
| Inara George | All Rise | | 00:27:42 - 00:33:25 (DVD) | When surgery is being performed on Cristina, |
| Maria Taylor | 11:11 | | 00:39:09 - 00:42:25 (DVD) | At the end of the episode. |
| 2x04 - Deny, Deny, Deny: |
| Ben Lee | Awake Is the New Sleep | | 00:00:30 - 00:02:20 (DVD) | At the beginning of the episode. |
| Roisin Murphy | Ruby Blue | | 00:26:02 - 00:27:30 (DVD) | When Derek and Christina talk about their patient and themselves. |
| The Delgados | Universal Audio | | 00:30:47 - 00:33:15 (DVD) | During Jeremiah's surgery. |
| Merrick | Merrick | | 00:38:47 - 00:42:25 (DVD) | At the end of the episode. |
| 2x05 - Bring The Pain: |
| Blue-Eyed Son | West of Lincoln | | 00:00:41 - 00:02:53 (DVD) | At the beginning of the episode. |
| Cowboy Racer | Yellow Horse EP | | 00:10:06 - 00:11:34 (DVD) | When Burke makes his way to the elevator. |
| Roisin Murphy | Ruby Blue | Prelude to Love In The Making | 00:22:44 - 00:24:36 (DVD) | When George starts surgery in the elevator. |
| Mike Doughty | Haughty Melodic | | 00:27:26 - 00:30:33 (DVD) | When George cross-clamps the aorta in the elevator. |
| Way Out West | Don't Look Now | | 00:31:15 - 00:36:01 (DVD) | When the shaman begins the soul finding ritual. |
| Tegan and Sara | If It Was You | | 00:38:27 - 00:42:21 (DVD) | At the end of the episode. |
| 2x06 - Into You Like A Train: |
| B.C. Camplight | Hide, Run Away | | 00:00:40 - 03:19:00 (DVD) | At the beginning of the episode. |
| The Hereafter | the hereafter | | 00:31:10 - 00:34:39 (DVD) | When the pole victims are talking to each other before their surgery. |
| The Umbrellas | Umbrellas | | 00:35:12 - 00:37:17 (DVD) | During the surgery of the pole victims. |
| Emilíana Torrini | Fisherman's Woman | | 00:39:20 - 00:42:25 (DVD) | At the end of this episode. |
| 2x07 - Something to Talk About: |
| The Faders | Plug in & Play | | 00:00:45 - 00:03:14 (DVD) | At the beginning of the episode. |
| Annie | Anniemal | | 00:17:20 - 00:19:50 (DVD) | When the interns are eating lunch. |
| Rosie Thomas | If Songs Could Be Held | | 00:23:15 - 00:25:21 (DVD) | When Addison and Derek meet up for lunch. |
| KT Tunstall | Eye To The Telescope | | 00:29:59 - 00:32:53 (DVD) | When Bailey stops Derek from talking to Meredith and Alex talks to Nicole about the first kiss. |
| Mark Joseph | Scream | | 00:37:39 - 00:42:22 (DVD) | At the end of the episode. |
| 2x08 - Let It Be: |
| The Chalets | Check In | | 00:00:31 - 00:02:05 (DVD) | At the beginning of the episode. |
| The Faders | Plug in & Play | | 00:13:14 - 00:15:21 (DVD) | When Cristina is picking a dress for her date with Burke. |
| Gabin | Mr. Freedom | Bang Bang To the Rock N Roll | 00:22:23 - 00:23:16 (DVD) | When the man in the restaraunt collapses. |
| Nouvelle Vague | Nouvelle Vague | | 00:30:26 - 00:31:57 (DVD) | When Burke and Cristina are performing surgery. |
| Joe Purdy | Julie Blue | | 00:34:40 - 00:36:42 (DVD) | When Derek talks to Weiss. |
| Anya Marina | Miss Halfway | | 00:39:30 - 00:42:24 (DVD) | At the end of the episode. |
| 2x09 - Thanks for the Memories: |
| Fannypack | See You Next Tuesday | | 00:00:00 - 00:00:23 (DVD) | At the beginning of the episode. |
| Dressy Bessy | Dressy Bessy | New Song (From Me To You) | 00:13:46 - 00:14:43 (DVD) | When Cristina calls George to find out where they keep the booze. |
| Mark McAdam | Boy Wonder | | 00:20:02 - 00:21:52 (DVD) | When Alex confesses to Meredith that he failed the medical boards. |
| The O.A.O.T's | Typical | | 00:24:10 - 00:25:32 (DVD) | When Burke and Izzie operate on the turkey and Joe and his boyfriend are taking bets. |
| Emilíana Torrini | Fisherman's Woman | | 00:35:35 - 00:38:01 (DVD) | When Bailey and Cristina perform the surgery on the patient that swallowed the wishbone. |
| Keren Ann | Not Going Anywhere | | 00:39:46 - 00:41:12 (DVD) | When George tells his dad about how he saved the man's life in the elevator. |
| Josh Rouse | Nashville | | 00:43:51 - 00:47:51 (DVD) | At the end of the episode. |
| 2x10 - Much Too Much: |
| Roisin Murphy | Ruby Blue | Night Of The Dancing Flame | 00:00:32 - 00:01:58 (DVD) | At the beginning of the episode. |
| Greens Keepers | Pleetch | | 00:15:20 - 00:18:48 (DVD) | When Cristina performs an enema on Steve. |
| Get Set Go | Ordinary World | | 00:31:39 - 00:33:58 (DVD) | When Cristina shows Burke her apartment. |
| Lifehouse | Lifehouse | | 00:34:27 - 00:37:46 (DVD) | When Olivia and Alex talk in the on-call room. |
| James Blunt | Back to Bedlam | | 00:39:45 - 00:42:25 (DVD) | At the end of the episode. |
| 2x11 - Owner of a Lonely Heart: |
| Jim Noir | Tower of Love | | 00:00:40 - 00:03:27 (DVD) | At the beginning of the episode. |
| Gemma Hayes | The Roads Don't Love You | | 00:20:33 - 00:23:11 (DVD) | When George and Olivia talk about Alex. |
| James Blunt | Back to Bedlam | | 00:28:45 - 00:30:23 (DVD) | When Meredith tries to apologize to Izzie. |
| Emilíana Torrini | Fisherman's Woman | | 00:35:11 - 00:36:40 (DVD) | When Meredith and Derek talk about his trailer. |
| Moonbabies | War on Sound | | 00:39:07 - 00:42:22 (DVD) | At the end of the episode. |
| 2x12 - Grandma Got Run Over By A Reindeer: |
| Nat 'King' Cole | The Christmas song | | 00:00:34 - 00:01:42 (DVD) | At the beginning of the episode. |
| Lou Rawls | Lou Rawls Christmas | Santa Claus Is Comin To Town | 00:06:01 - 06:41:00 (DVD) | When Burke and Cristina tell Justin and Mariann the great news. |
| Mascott | | | 00:06:43 - 00:09:01 (DVD) | When Burke tells Justin he needs the new heart. |
| Jet | Rare Tracks | | 00:16:51 - 00:18:28 (DVD) | When Alex is examining Cristina and George walks in. |
| Maria Taylor | | | 00:20:36 - 00:22:44 (DVD) | When Derek and Izzie talk about the patient, Tim Epstein. |
| Holidays on Ice | Playing Boyfriends & Girlfriends | | 00:27:12 - 00:30:49 (DVD) | When Derek and Mrs. Epstein talk. |
| The LeeVees | Hanukkah Rocks | | 00:31:43 - 00:32:52 (DVD) | When Bailey, George, and Meredith perform the gastric ulcer surgery on Nadia Shelton. |
| Catie Curtis | | Lo, How A Rose E'er Blooming | 00:33:20 - 00:35:10 (DVD) | When Cristina talks to Justin. |
| Sixpence None The Richer | A Winter's Night | It Came Upon A Midnight Clear | 00:38:08 - 00:42:18 (DVD) | At the end of the episode. |
| Special - Straight to the Heart: |
| (None known) | | | | |
| 2x13 - Begin the Begin: |
| The 88 | Over and Over | | 00:00:32 - 00:02:40 (DVD) | At the beginning of the episode. |
| Kraak & Smaak | Boogie Angst | | 00:13:01 - 00:14:40 (DVD) | When Meredith and Derek talk in the elevator. |
| Cary Brothers | The Last Kiss | | 00:23:45 - 00:25:52 (DVD) | When Addison and George talk to Mr. and Mrs. Singleton about Bex's surgery. |
| Slow Runner | No Disassemble | | 00:38:53 - 00:42:18 (DVD) | At the end of the episode. |
| 2x14 - Tell Me Sweet Little Lies: |
| St. Etienne | Tales from Turnpike House | | 00:00:29 - 00:02:08 (DVD) | At the beginning of the episode. |
| Metric | Live It Out | | 00:03:03 - 00:03:48 (DVD) | When the musician is rushed in. |
| Mike Tarantino | | | 00:14:38 - 00:15:35 (DVD) | When Cristina, Alex, and George have the hot dog eating contest. |
| Sam Winch | The Lullabadeer | | 00:31:16 - 00:33:21 (DVD) | When Sophie and George talk about the nursing home. |
| Carey Ott | Lucid Dream | | 00:33:23 - 00:35:46 (DVD) | When Alex and Izzie tell Yumi she's going to be fine. |
| Adrienne Pierce | Faultline | | 00:39:01 - 00:41:56 (DVD) | At the end of the episode. |
| 2x15 - Break on Through: |
| Aqueduct | I Sold Gold | | 00:00:32 - 00:02:44 (DVD) | At the beginning of the episode. |
| Vassy | My Affection | | 00:21:49 - 00:23:37 (DVD) | When the nurses celebrate in the bar with O'Malley. |
| Kate Earl | Fate Is the Hunter | | 00:24:40 - 00:27:17 (DVD) | When Cristina tells her patient her secret. |
| Landon Pigg | LP | | 00:31:50 - 00:33:59 (DVD) | When Meredith removed the tube from her patient. |
| Kendall Payne | Grown | | 00:34:01 - 00:38:26 (DVD) | When surgery is being performed on Lizzys patient. |
| Leeroy Stagger | Beautiful House | | 00:40:55 - 00:42:23 (DVD) | At the end of the episode. |
| 2x16 - It's the End of the World (1): |
| The Monotypes | Dead Streets | | 00:00:27 - 00:01:41 (DVD) | At the beginning of the episode. |
| KT Tunstall | Eye To The Telescope | | 00:14:37 - 00:16:14 (DVD) | While Burke and Derek continue their first-name discussion. |
| Ursula 1000 | Here Comes Tomorrow | | 00:31:43 - 00:32:44 (DVD) | While Izzy and Alex are undressing in the room. |
| Michael Stipe | In the Sun EP | | 00:39:26 - 00:42:24 (DVD) | At the end of the episode. |
| 2x17 - As We Know It (2): |
| Correatown | | | 00:01:22 - 00:02:40 (DVD) | At the beginning of the episode. |
| Ambulance LTD | LP | | 00:05:30 - 00:06:27 (DVD) | When BIrke and Meredith send Cristina away. |
| The Weepies | Say I Am You | | 00:09:40 - 00:12:03 (DVD) | When Izzy and Alex make out. |
| Greenskeepers | Pleetch | | 00:19:29 - 00:22:57 (DVD) | When they move the gurney. |
| Anna Nalick | Wreck of the Day | | 00:28:32 - 00:33:50 (DVD) | When Meredith prepares to pull out the bomb. |
| Kate Havnevik | Unlike Me [CD-SINGLE] | | 00:36:54 - 00:39:04 (DVD) | When Izzy and Cristina are cleaning Meredith. |
| Foy Vance | Watermelon Oranges [CD-SINGLE] | | 00:41:34 - 00:42:18 (DVD) | At the end of the episode. |
| 2x18 - Yesterday: |
| Diplo | Florida | | 00:00:29 - 00:01:53 (DVD) | At the beginning of the episode. |
| The Chalets | Check In | | 00:18:42 - 00:19:54 (DVD) | While Derek and Mark argue in Dr. Webbers office. |
| Devics | Push The Heart | | 00:20:23 - 00:22:32 (DVD) | When Addison meets Mark on the bridge. |
| Joshua Radin | We Were Here | | 00:26:11 - 00:29:00 (DVD) | When Izzy and Pamela talk about her "episodes". |
| Rosie Thomas | If Songs Could Be Held | It Don't Matter to the Sun | 00:30:48 - 00:33:13 (DVD) | When Meredith confronts Thatcher. |
| Headlights | The Enemies EP | Everybody Needs A Fence To Lean On | 00:33:15 - 00:35:35 (DVD) | When Derek, Addison and Marc meet in the elevator. |
| Corinne Bailey Rae | Corinne Bailey Rae | | 00:36:57 - 00:40:51 (DVD) | After Burke shouts at Cristina. |
| 2x19 - What Have I Done To Deserve This?: |
| Madeleine Peyroux | Careless Love | | 00:00:43 - 00:02:47 (DVD) | At the beginning of the episode. |
| Get Set Go | Ordinary World | | 00:25:31 - 00:28:24 (DVD) | After George fell down the stairs. |
| Boy Least Likely To | The Best Party Ever | | 00:30:02 - 00:32:02 (DVD) | When Izzy and Denny talk. |
| Brandi Carlile | Brandi Carlile | | 00:34:21 - 00:36:54 (DVD) | When George packs his suitcase. |
| New Moscow | Verse Chorus Worse | | 00:38:25 - 00:42:31 (DVD) | At the end of the episode. |
| 2x20 - Band Aid Covers the Bullet Hole: |
| Sing Sing | Sing-Sing and I | | 00:00:35 - 00:02:49 (DVD) | At the beginning of the episode. |
| The Last Town Chorus | Wire Waltz | | 00:14:17 - 00:17:03 (DVD) | While Derek and Addison check the size of the aneurysm. |
| The Urbs | Toujours le Meme Film | | 00:21:24 - 00:22:54 (DVD) | When the interns are eating lunch. |
| Tina Dico | In the Red | | 00:33:00 - 00:34:40 (DVD) | When Bailey takes back her baby. |
| Jem | Finally Woken | | 00:38:34 - 00:42:14 (DVD) | At the end of the episode. |
| 2x21 - Superstition: |
| Greenskeepers | Pleetch | | 00:00:39 - 00:02:29 (DVD) | At the beginning of the episode. |
| Anna Nalick | Breathe (2 A.M.) [CD-SINGLE] | Catalyst (Acoustic Version) | 00:14:57 - 00:16:47 (DVD) | When Ollie and Dr. Webber talk. |
| Luke Doucet | Broken (And Other Rogue States) | | 00:23:48 - 00:25:30 (DVD) | When the interns are eating lunch. |
| The Fray | How To Save A Life | | 00:33:20 - 00:35:30 (DVD) | During all the operations. |
| Foy Vance | Gabriel and the Vagabond [Single] | | 00:35:31 - 00:38:52 (DVD) | When Izzy tries to find out who died. |
| Jamie Lidell | Multiply | | 00:39:40 - 00:42:18 (DVD) | At the end of the episode. |
| 2x22 - The Name of the Game: |
| Dressy Bessy | Dressy Bessy | | 00:00:31 - 00:03:44 (DVD) | At the beginning of the episode. |
| Gnarls Barkley | St. Elsewhere | | 00:18:08 - 00:19:43 (DVD) | When the interns are eating lunch. |
| The Sunshine | Love | | 00:24:45 - 00:26:40 (DVD) | After Meredith talked to her step-sister. |
| Anna Nalick | Wreck Of The Day | Wreck Of The Day (Acoustic) | 00:29:03 - 00:33:48 (DVD) | When Dr. Webber and Thatcher talk. |
| Joshua Radin | | | 00:39:19 - 00:42:21 (DVD) | At the end of the episode. |
| Special - Under Pressure: |
| (None known) | | | | |
| 2x23 - Blues for Sister Someone: |
| The Sunshine | Love | | 00:00:33 - 00:01:46 (DVD) | At the beginning of the episode. |
| Metric | Live It Out | | 00:22:16 - 00:24:35 (DVD) | When Meredith and Derek talk in the lift about bad sex. |
| James Morrison | Undiscovered | | 00:29:08 - 00:31:38 (DVD) | When Meredith and Finn run an errand before their date. |
| Hotel Lights | Hotel Lights | | 00:37:10 - 00:42:21 (DVD) | At the end of the episode. |
| 2x24 - Damage Case: |
| Sergio Belem | | | 00:00:30 - 00:02:29 (DVD) | At the beginning of the episode. |
| Inara George | | | 00:21:30 - 00:23:23 (DVD) | When Baily and Addison explain Melanie’s parents her daughter’s condition. |
| Brandi Carlile | Brandi Carlile | | 00:30:11 - 00:34:14 (DVD) | When Melanie is having the second surgery. |
| Kate Havnevik | Melankton | | 00:34:14 - 00:38:16 (DVD) | While Marshall apologizes with Jim for causing the accident. |
| Regina Spektor | Begin to Hope | | 00:38:28 - 00:42:11 (DVD) | At the end of the episode. |
| 2x25 - 17 Seconds: |
| Yeah Yeah Yeahs | Show Your Bones | | 00:00:36 - 00:02:00 (DVD) | At the beginning of the episode. |
| Snow Patrol | Final Straw | Somewhere A Clock Is Ticking | 00:37:32 - 00:42:16 (DVD) | At the end of the episode. |
| 2x26 - Deterioration of the Fight or Flight Response: |
| KT Tunstall | Eye To The Telescope | | 00:19:00 - 00:21:27 (DVD) | When Derek tries to hide in a room and talks with Dr. Webber instead. |
| The Coral Sea | Volcano and Heart | | 00:32:29 - 00:34:34 (DVD) | When Dr. Hahn and Alex arrive at Seattle Grace by helicopter. |
| Au Revoir Simone | Verses of Comfort, Assurance & Salvation | | 00:34:35 - 00:36:00 (DVD) | When Dr. Hahn performs Denny's heart transplant. |
| Gomez | How We Operate | | 00:39:39 - 00:42:10 (DVD) | At the end of part 1, when Derek must perform a wake-up test. |
| 2x27 - Losing My Religion: |
| Pete Droge | Under the Waves | | 00:58:49 - 01:00:10 (DVD) | When Derek and Addison talk. |
| Dressy Bessy | Electrified | | 01:13:25 - 01:14:37 (DVD) | First song at the prom when Meredith arrives. |
| Amos Lee | Amos Lee | | 01:14:37 - 01:15:50 (DVD) | Second song at the prom when Dr. Webber dances with his niece. |
| Masha Qrella | Unsolved Remained | | 01:16:58 - 01:19:12 (DVD) | Third song at the prom when Meredith and Finn dance. |
| Kate Havnevik | | | 01:20:33 - 01:23:15 (DVD) | When Derek and Meredith are in the operating room. |
| Snow Patrol | Eyes Open | | 01:26:27 - 01:31:20 (DVD) | At the end of the episode. |